LDHA Mouse Monoclonal Antibody [20-179]
Catalog# M1007-7
LDHA Mouse Monoclonal Antibody [20-179]
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IHC-P
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Human
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Mouse
概述
产品名称
LDHA Mouse Monoclonal Antibody [20-179]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human LDHA aa 1-50 / 332.
种属反应性
Human, Mouse
验证应用
IHC-P
分子量
Predicted band size: 37 kDa
阳性对照
Mouse testis tissue, human kidney tissue.
偶联
unconjugated
克隆号
20-179
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:50-1:100
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
靶点
功能
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.
背景文献
1. Singer P.A., Trevor K., Oshima R.G. J. Biol. Chem. 261:538-547(1986).
2. Ichinose Y., Morita T., Zhang F. etc. Gene 70:85-95(1988).
3. Dixon M.J., Robinson V., White A., Ferguson M.W. J. Anat. 183:423-438(1993).
序列相似性
Belongs to the LDH/MDH superfamily. LDH family.
翻译后修饰
ISGylated.
亚细胞定位
Cytoplasm
别名
Cell proliferation-inducing gene 19 protein antibody
GSD11 antibody
L lactate dehydrogenase A chain antibody
L-lactate dehydrogenase A chain antibody
l7R2 antibody
Lactate dehydrogenase 1, A chain antibody
Lactate dehydrogenase A antibody
Lactate dehydrogenase A4 antibody
Lactate dehydrogenase M antibody
LDH A antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LDHA antibody (M1007-7) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Palmitoylation of NOD1 and NOD2 is required for bacterial sensing
Author: Gregory D. Fairn,Brian Raught, Qiming Sun,Dante Neculai
PMID: 31649195
应用: WB
反应种属: Human
发表时间: 2019 Oct
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Citation
Alternative Products
Lactate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [SU39-06]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,IP
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
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