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JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

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Catalog# ET1607-35

JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within C-terminal human JAK2.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell

分子量

Predicted band size: 131 kDa

阳性对照

TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3.

偶联

unconjugated

克隆号

SY0245

RRID

AB_3069760

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100-1:500

发表文章中的应用

WB 查看 10 篇文献如下

发表文章中的种属

Mouse 查看 5 篇文献如下
Human 查看 3 篇文献如下
Human，Mouse 查看 1 篇文献如下

靶点

功能

JAK2 (Janus kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

背景文献

1. Gan, X.T. et al. 2015. Myocardial Hypertrophic Remodeling and Impaired Left Ventricular Function in Mice with a Cardiac-Specific Deletion of Janus Kinase 2. The American journal of pathology. 185: 3202-10.

2. Tang, H. et al. 2011. Changes in growth hormone (GH), GH receptor, and GH signal transduction in hippocampus of congenital hypothyroid rats. J. Neurosci. Res. 89: 248-255.

序列相似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

组织特异性

Ubiquitously expressed throughout most tissues.

翻译后修饰

Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.

亚细胞定位

Endomembrane system, Cytoplasm, Nucleus.

UNIPROT

SwissProt: O60674 Human

SwissProt: Q62120 Mouse

SwissProt: Q62689 Rat

别名

JAK 2 antibody

JAK-2 antibody

JAK2 antibody

JAK2_HUMAN antibody

Janus Activating Kinase 2 antibody

Janus kinase 2 (a protein tyrosine kinase) antibody

Janus kinase 2 antibody

JTK 10 antibody

JTK10 antibody

kinase Jak2 antibody

展开

图片

  • Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution.<br /><br />Lane 1: TF-1 cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 5 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

    Lane 1: TF-1 cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 120 kDa

    Exposure time: 5 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/500 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: MEF cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 2 minutes;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/500 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: MEF cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 120 kDa

    Exposure time: 2 minutes;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si JAK2 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 45 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-35" style="font-weight: bold;text-decoration: underline;">ET1607-35</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si JAK2 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 120 kDa

    Exposure time: 45 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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