IL-7 Mouse Monoclonal Antibody [A8B11]
Catalog# HA601048
IL-7 Mouse Monoclonal Antibody [A8B11]
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WB
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Human
概述
产品名称
IL-7 Mouse Monoclonal Antibody [A8B11]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human IL-7 aa 1-177/177.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 20 kDa
阳性对照
HepG2 cell lysates.
偶联
unconjugated
克隆号
A8B11
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
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1:500
靶点
功能
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19).
背景文献
1. Sun S. et. al. Gene fusion of IL7 involved in the regulation of idiopathic pulmonary fibrosis. Ther Adv Respir Dis. 2021 Jan-Dec
2. Perez-Witzke D. et. al. Development of a long-term, IL7 dependent cell death rescue assay in CD4+ T-cells. J Immunol Methods. 2021 Jun
亚细胞定位
Secreted.
UNIPROT
别名
IL 7 antibody
IL-7 antibody
Il7 antibody
IL7_HUMAN antibody
Interleukin 7 antibody
Interleukin-7 antibody
LP-1 antibody
Lymphopoietin 1 antibody
图片
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Western blot analysis of IL-7 on HepG2 cell lysates with Mouse anti-IL-7 antibody (HA601048) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 2 minutes;
15 % SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601048) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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