IL-4 Mouse Monoclonal Antibody [A7D7]
Catalog# HA601034
IL-4 Mouse Monoclonal Antibody [A7D7]
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ELISA
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Human
概述
产品名称
IL-4 Mouse Monoclonal Antibody [A7D7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human aa 20-153 / 153.
种属反应性
Human
验证应用
ELISA
分子量
Predicted band size: 17 kDa
偶联
unconjugated
克隆号
A7D7
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA
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1:20,000
靶点
功能
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
背景文献
1. Nguyen JK. et. al. The IL-4/IL-13 axis in skin fibrosis and scarring: mechanistic concepts and therapeutic targets. Arch Dermatol Res. 2020 Mar
2. Matsunaga MC. et. al. IL-4 and IL-13 Inhibition in Atopic Dermatitis. J Drugs Dermatol. 2016 Aug
亚细胞定位
Secreted.
UNIPROT
别名
B cell growth factor 1 antibody
B cell IgG differentiation factor antibody
B Cell Stimulatory Factor 1 antibody
B-cell stimulatory factor 1 antibody
BCGF 1 antibody
BCGF1 antibody
Binetrakin antibody
BSF-1 antibody
BSF1 antibody
IGG1 induction factor antibody
展开图片
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IL-4 Antibody (HA601034) in indirect ELISA.
Indirect ELISA analysis of IL-4 was performed by coating wells of a 96-well plate with 50 µl per well of IL-4 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse IL-4 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
IL-4 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human
Conjugate: unconjugated
