Human SIRP alpha/CD172a Protein
Catalog# HA210252
Human SIRP alpha/CD172a Protein
Product Information
Product name
Human SIRP alpha/CD172a Protein
Catalog No.
HA210252
Predicted molecular mass
The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.
Protein construction description
Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu31-Arg370.
Accession
P78324-1
Source
HEK293
Purity
> 95% as determined by Tris-Bis PAGE > 95% as determined by SEC-HPLC
Endotoxin
Less than 1EU per μg by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species
Human
Background
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Usage guide
Storage
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
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