Hsp90 alpha Mouse Monoclonal Antibody [A10-A5-D2]
Catalog# M1603-3
Hsp90 alpha Mouse Monoclonal Antibody [A10-A5-D2]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Hsp90 alpha Mouse Monoclonal Antibody [A10-A5-D2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human Hsp90 alpha aa 1-50 / 732.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 90 kDa
阳性对照
HeLa cell lysate, 293T cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, HeLa, NIH/3T3, PC-12, human kidney tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
克隆号
A10-A5-D2
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:500
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IHC-P
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1:1,000
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FC
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1:1,000
靶点
功能
The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.
背景文献
1. Zhang Y et al. A systems biology-based investigation into the therapeutic effects of Gansui Banxia Tang on reversing the imbalanced network of hepatocellular carcinoma. Sci Rep 4:4154 (2014).
2. Barabutis N et al. LPS induces pp60c-src-mediated tyrosine phosphorylation of Hsp90 in lung vascular endothelial cells and mouse lung. Am J Physiol Lung Cell Mol Physiol 304:L883-93 (2013).
序列相似性
Belongs to the heat shock protein 90 family.
翻译后修饰
ISGylated.; S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.; Ubiquitinated via 'Lys-63'-linked polyubiquitination by HECTD1. Ubiquitination promotes translocation into the cytoplasm away from the membrane and secretory pathways.
亚细胞定位
Nucleus, Cytoplasm, Melanosome, Cell membrane, Mitochondrion.
别名
EL52 antibody
epididymis luminal secretory protein 52 antibody
Heat shock 86 kDa antibody
heat shock 90kD protein 1, alpha antibody
Heat shock 90kD protein 1, alpha like 4 antibody
heat shock 90kD protein, alpha-like 4 antibody
Heat shock 90kDa protein 1 alpha antibody
Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
Heat shock protein HSP 90-alpha antibody
HS90A_HUMAN antibody
展开图片
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Western blot analysis of Hsp90 alpha on different lysates with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lane 4: NIH/3T3 cell lysate -
Immunocytochemistry analysis of HeLa cells labeling Hsp90 alpha with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling Hsp90 alpha with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling Hsp90 alpha with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Hsp90 alpha antibody (M1603-3) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1603-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling Hsp90 alpha.
Cells were fixed and permeabilized. Then stained with the primary antibody (M1603-3, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Pharmacological investigation of indole alkaloids from Alstonia scholaris against chronic glomerulonephritis
期刊: Phytomedicine
DOI:
IF: 7.9
应用: WB
反应种属: Mouse
发表时间: 2023 Jul
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Conserved Mitotic Phosphorylation of a Proteasome Subunit Regulates Cell Proliferation
期刊: Cells
DOI:
IF: 6.600
应用: WB
反应种属: Human
发表时间: 2021 Nov
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