Histone H4 Mouse Monoclonal Antibody [H2-E7]
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Catalog# EM1706-19
Histone H4 Mouse Monoclonal Antibody [H2-E7]
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WB
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IF-Cell
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IHC-P
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FC
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Human
概述
产品名称
Histone H4 Mouse Monoclonal Antibody [H2-E7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
11.4 kDa
阳性对照
THP-1 cell lysate, Hela, paraffin-embedded rectum cancer tissues, paraffin-embedded stomach cancer tissues, Raji.
偶联
unconjugated
克隆号
H2-E7
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:200-1:500
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IHC-P
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1:200-1:500
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FC
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1:100-1:200
靶点
功能
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
背景文献
1. Quarato G et al. Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding Sites Executes Necroptosis. Mol Cell N/A:N/A (2016).
2. Sako K et al. Ky-2, a Histone Deacetylase Inhibitor, Enhances High-Salinity Stress Tolerance in Arabidopsis thaliana. Plant Cell Physiol 57:776-83 (2016).
亚细胞定位
Nucleus. Chromosome.
UNIPROT
别名
dJ160A22.1 antibody
dJ160A22.2 antibody
dJ221C16.1 antibody
dJ221C16.9 antibody
FO108 antibody
H4 antibody
H4.k antibody
H4/a antibody
H4/b antibody
H4/c antibody
展开图片
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Western blot analysis of Histone H4 on THP-1 cell lysate using anti- Histone H4 antibody at 1/1,000 dilution.
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ICC staining Histone H4 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Histone H4 antibody.
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Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti- Histone H4 antibody.
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Flow cytometric analysis of Raji cells with Histone H4 antiboy at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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