HDAC2 Rabbit Polyclonal Antibody
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Catalog# R1601-7
HDAC2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
概述
产品名称
HDAC2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within C-terminal human Histone Deacetylase 2.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 55 kDa
阳性对照
SH-SY5Y cell lysate, 293T cell lysate, Hela cell lysate, PC-12 cell lysate, Wild-type Hela whole cell lysate, LOVO, NIH/3T3, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:500-1:2,000
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IHC-P
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1:100-1:200
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FC
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1:50-1:100
靶点
功能
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.
背景文献
暂无
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily.
组织特异性
Widely expressed; lower levels in brain and lung.
翻译后修饰
S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
亚细胞定位
Nucleus, Cytoplasm.
别名
D10Wsu179e antibody
HD 2 antibody
HD2 antibody
HDAC 2 antibody
Hdac2 antibody
HDAC2_HUMAN antibody
Histone deacetylase 2 (HD2) antibody
Histone deacetylase 2 antibody
OTTHUMP00000017046 antibody
OTTHUMP00000227077 antibody
展开图片
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Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y cell lysate
Lane 2: 293T cell lysate
Lane 3: Hela cell lysate
Lane 4: PC-12 cell lysate -
☑ Knockout (KO)
All lanes: Western blot analysis of HDAC2 with anti-HDAC2 antibody (R1601-7) at 1/500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: HDAC2 knockout Hela whole cell lysate (10 µg).
R1601-7 was shown to specifically react with HDAC2 in wild-type Hela cells. No bands were observed when HDAC2 knockout sample were tested. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1601-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining HDAC2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
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Conjugate: unconjugated
HDAC2 Recombinant Rabbit Monoclonal Antibody [SD0816]
Application: WB,IP,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点 & 同通路的产品
HDAC2 Recombinant Rabbit Monoclonal Antibody [SY29-02]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated