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HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]

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Catalog# HA601176

HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]

抗体类型

Recombinant Mouse Monoclonal Antibody

免疫原

Synthetic peptide within Human HSP90 Beta aa 311-360 / 724.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF-Cell

分子量

Predicted band size: 83.3 kDa

阳性对照

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, HeLa.

偶联

unconjugated

克隆号

2-1-G3-R

RRID

AB_3071895

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:5,000

  • IF-Cell

  • 1:100

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression.

背景文献

1. Chadli A., Graham J.D., Abel M.G., Jackson T.A., Gordon D.F., Wood W.M., Felts S.J., Horwitz K.B., Toft D. GCUNC-45 is a novel regulator for the progesterone receptor/hsp90 chaperoning pathway. Mol. Cell. Biol. 26:1722-1730 (2006)

2. Verma S., Goyal S., Jamal S., Singh A., Grover A. Hsp90: Friends, clients and natural foes. Biochimie 127:227-240 (2016)

亚细胞定位

Cytoplasm

UNIPROT

SwissProt: P08238 Human

SwissProt: P11499 Mouse

SwissProt: P34058 Rat

别名

90 kda heat shock protein beta HSP90 beta antibody

D6S182 antibody

FLJ26984 antibody

Heat shock 84 kDa antibody

Heat shock 90kD protein 1, beta antibody

Heat shock 90kDa protein 1 beta antibody

Heat shock protein 90 alpha family class B member 1 antibody

Heat shock protein 90 kDa antibody

Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody

Heat shock protein 90kDa alpha family class B member 1 antibody

展开

图片

  • Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: C6 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 10: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 11: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 83 kDa<br /><br />Exposure time: 53 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (HA601176) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: C6 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: Mouse brain tissue lysate (40 µg/Lane)
    Lane 10: Mouse heart tissue lysate (40 µg/Lane)
    Lane 11: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

    Exposure time: 53 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601176) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (HA601176) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601176) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (HA601176) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601176) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of HeLa cells labeling HSP90 Beta with Mouse anti-HSP90 Beta antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 Beta antibody (<a href="/products/HA601176" style="font-weight: bold;text-decoration: underline;">HA601176</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling HSP90 Beta with Mouse anti-HSP90 Beta antibody (HA601176) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 Beta antibody (HA601176) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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