Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Rmb: 5600
Catalog# HA610286
Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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IHC-Fr
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WB
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Mouse
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Rat
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HA601452
含抗保成分
-
unconjugated
概述
产品名称
Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1,409.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, WB
靶点分子量
Predicted band size: 165 kDa
阳性对照
Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate.
偶联
unconjugated
克隆号
PSH07-76
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). The receptor recognises terminal mannose, N-acetylglucosamine and fucose residues on glycans attached to proteins found on the surface of some microorganisms, playing a role in both the innate and adaptive immune systems. Additional functions include clearance of glycoproteins from circulation, including sulphated glycoprotein hormones and glycoproteins released in response to pathological events. The mannose receptor recycles continuously between the plasma membrane and endosomal compartments in a clathrin-dependent manner.
背景文献
1. Nielsen MC et al. Macrophage Activation Markers, CD163 and CD206, in Acute-on-Chronic Liver Failure. Cells. 2020 May
2. Nawaz A et al. Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration. Nat Commun. 2022 Nov
亚细胞定位
Endosome membrane, Cell membrane.
别名
bA541I19.1 antibody
C type lectin domain family 13 member D antibody
C-type lectin domain family 13 member D antibody
CD 206 antibody
CD206 antibody
CD206 antigen antibody
CLEC13D antibody
CLEC13DL antibody
Macrophage mannose receptor 1 antibody
Macrophage mannose receptor 1 like protein 1 antibody
展开图片
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This data was developed using HA601452, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601452, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601452, the same antibody clone in a different buffer formulation.
Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (HA601452) at 1/2,000 dilution.
Lane 1: Mouse lung tissue lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 200 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601452) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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