Skip to content

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric)

Rmb: 1500

产品规格

点击询价

联系我们

邮箱: market@huabio.cn

技术支持电话：0571-86052305

0571-89986345

市场客服电话：0571-88062880

查找各地代理商

Catalog# HA601427

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric)

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC(Intra)

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within N-terminal human alpha smooth muscle Actin.

产品特异性

Rat secondary antibodies are required for detection. This product has cross-reactions with rabbit secondary antibodies.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P, FC(Intra), mIHC

靶点分子量

Predicted band size: 42 kDa

阳性对照

Saos-2 (Human osteosarcoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate.

偶联

unconjugated

克隆号

SY02-64

反应性数据

WB IF-Cell IHC-P FC(Intra) mIHC
Human
Tested Tested
Not Recommended
Not Recommended
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IF-Cell

  • 1:5,000

  • IHC-P

  • 1:50,000

  • FC(Intra)

  • 1:1,000

  • mIHC

  • 1:3,000

靶点

功能

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

背景文献

1. Izumi D et al. CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin 1 clustering and invasiveness in gastric cancer. Int J Cancer 138:1207-19 (2016).

2. Chung SI et al. Development of a transgenic mouse model of hepatocellular carcinoma with a liver fibrosis background. BMC Gastroenterol 16:13 (2016).

序列相似性

Belongs to the actin family.

翻译后修饰

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.; Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-75 by SETD3.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

亚细胞定位

Cytoplasm.

UNIPROT

SwissProt: P62736 Human

SwissProt: P62737 Mouse

SwissProt: P62738 Rat

别名

alpha SMA

a-SMA antibody

asma antibody

a actin antibody

AAT6 antibody

ACTA_HUMAN antibody

ACTA2 antibody

Actin alpha 2 smooth muscle aorta antibody

Actin aortic smooth muscle antibody

Actin, aortic smooth muscle antibody

展开

图片

  • Western blot analysis of alpha smooth muscle Actin on different lysates with Rat anti-alpha smooth muscle Actin antibody (<a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a>) at 1/5,000 dilution.<br /><br />Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate<br />Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 4: C2C12 (Mouse myoblast) cell lysate<br />Lane 5: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate<br />Lane 6: Mouse skin tissue lysate<br />Lane 7: Rat skin tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rat IgG-HRP (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa

    Western blot analysis of alpha smooth muscle Actin on different lysates with Rat anti-alpha smooth muscle Actin antibody (HA601427) at 1/5,000 dilution.

    Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate
    Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 4: C2C12 (Mouse myoblast) cell lysate
    Lane 5: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
    Lane 6: Mouse skin tissue lysate
    Lane 7: Rat skin tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601427, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse<br />Sample: NIH/3T3 (Mouse fibroblast)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br /><br />Primary antibody: <a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a>, 1/5,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature.<br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601427, 1/5,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Small intestine<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a>, 1/50,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Small intestine
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601427, 1/50,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 20 minutes at room temperature.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Human<br />Sample: Saos-2 (Human osteosarcoma cell)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a> (1/1,000, Red), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: Saos-2 (Human osteosarcoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA601427 (1/1,000, Red), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Mouse<br />Sample: NIH/3T3 (Mouse fibroblast)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA601427" style="font-weight: bold;text-decoration: underline;">HA601427</a> (1/1,000, Red), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA601427 (1/1,000, Red), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

同靶点 & 同通路的产品

metafield image

alpha smooth muscle Actin Recombinant Antibody - Rat IgG1 (Chimeric)

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03]

Application: WB,IHC-P,FC,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Antibody - Mouse IgG1 (Chimeric)

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Mouse IgG1 (Chimeric)

Application: WB,IHC-P,IF-Cell,FC,mIHC,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application: WB,IHC-P,IF-Cell,FC,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric) - BSA and Azide free

Application: WB,IF-Cell,IHC-P,FC(Intra)

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

Copyright @ 2026 HUABIO

网站备案号：  浙ICP备09005892号-1 |  浙公网安备 33019202000643号

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

HI！我是 LUMI，可以帮您解决产品和实验问题哦～