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Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647)

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Catalog# 003-301-009

Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647)

Application

  • WB

  • ICC/IF

  • ELISA

  • Flow Cyt

Reactivity

  • Species independent

Conjugation

  • iFluor™ 647

概述

产品名称

Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647)

抗体类型

Alpaca Nanobody

免疫原

HA tag fused KLH

产品特异性

HA tag(YPYDVPDYA)

种属反应性

Species independent

验证应用

WB, ICC/IF, ELISA, Flow Cyt

偶联

iFluor™ 647

产品特性

形态

Liquid

浓度

存放说明

Store at –20 ℃(Avoid freeze / thaw cycles), Protect from light.

存储缓冲液

10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol.

亚型

VHH domain of alpaca IgG2b/2c fused to Mouse IgG2a Fc(mutation)

纯化方式

Recombinant Expression and Affinity purified.

应用稀释度

  • WB

  • 1:5,000-1:20,000

  • ELISA

  • 1:5,000-1:20,000

  • ICC/IF

  • 1:200-1:1,000

  • Flow Cyt

  • 1µg for 10^6 cells

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

靶点

功能

Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is based on monoclonal, recombinant, mouse IgG2a Fc fused single domain antibody to HA tag coupled to iFluor647. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpHcAbs® Mouse IgG2a antibody(iFluor647) detects the HA tag selectively, no reactivity with other proteins.

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