Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]
Catalog# HA721989
Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]
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WB
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Human
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Mouse
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Rat
概述
产品名称
Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Glutathione Reductase aa 473-522 / 522.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 56 kDa
阳性对照
HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate.
偶联
unconjugated
克隆号
JE31-42
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
靶点
功能
Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.
背景文献
1. Xu FL et al. SLC27A5 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by downregulating glutathione reductase. Cell Death Dis. 2023 Jan
2. Ehtiati S et al. Glutathione reductase system changes in HTLV-1 infected patients. Virusdisease. 2022 Mar
亚细胞定位
Mitochondrion; Cytoplasm.
别名
GLR1 antibody
GLUR antibody
Glutathione reductase antibody
Glutathione reductase mitochondrial antibody
Glutathione reductase, mitochondrial antibody
GR antibody
Gr1 antibody
GRase antibody
GRD 1 antibody
GRD1 antibody
展开图片
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Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: Mouse liver tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
Lane 8: Rat lung tissue lysate (40 µg/Lane)
Lane 9: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (HA721989) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Glutathione Reductase cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 54 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"