Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PSH10-87]
Catalog# HA723254
Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PSH10-87]
-
WB
-
Human
概述
产品名称
Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PSH10-87]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Gasdermin D aa 226-275.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 30 kDa
阳性对照
THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate.
偶联
unconjugated
克隆号
PSH10-87
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
靶点
功能
Gasdermin D (GSDMD, from combination of gastro and dermato, referencing the locations where its family of proteins were originally found to be primarily expressed is a protein that in humans is encoded by the GSDMD gene on chromosome 8. Several current studies have revealed that GSDMD serves as a specific substrate of inflammatory caspases (caspase-1, -4, -5 and -11) and as an effector molecule for the lytic and highly inflammatory form of programmed cell death known as pyroptosis. Hence, GSDMD is an essential mediator of host defence against microbial infection and danger signals. The pore-forming activity of the N-terminal cleavage product causes cell swelling and lysis to prevent intracellular pathogens from replicating, and is required for the release of cytoplasmic content such as the inflammatory cytokine interleukin-1β (IL-1β) into the extracellular space to recruit and activate immune cells to the site of infection. GSDMD has an additional potential role as an antimicrobial by binding to cardiolipin (CL) and form pores on bacterial membranes.
背景文献
1. Wei C et al. Brain endothelial GSDMD activation mediates inflammatory BBB breakdown. Nature. 2024 May
2. Li Z et al. The Regulation and Modification of GSDMD Signaling in Diseases. Front Immunol. 2022 Jun
亚细胞定位
Cell membrane, Secreted, Mitochondrion membrane, Cytoplasm, cytosol.
UNIPROT #
别名
1810036L03Rik antibody
DF 5L antibody
DF5L antibody
DFNA 5L antibody
DFNA5L antibody
FKSG 10 antibody
FKSG10 antibody
FLJ12150 antibody
Gasdermin antibody
Gasdermin D antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA723254) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723254) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
Gasdermin D (N terminal) Rabbit Polyclonal Antibody
Application: WB,IHC-P,FC,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
