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GSDMD Mouse Monoclonal Antibody [A8D7]

RMB: 900 特惠 1500

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Catalog# HA601046

GSDMD Mouse Monoclonal Antibody [A8D7]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GSDMD Mouse Monoclonal Antibody [A8D7]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human GSDMD aa 101-300/484.

种属反应性

Human, Mouse

验证应用

WB

分子量

Predicted band size: 53 kDa

阳性对照

PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate.

偶联

unconjugated

克隆号

A8D7

RRID

AB_3073484

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG2b

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500

靶点

功能

Gasdermin D (GSDMD) is a protein that in humans is encoded by the GSDMD gene on chromosome 8. It belongs to the gasdermin family which is conserved among vertebrates and comprises six members in humans, GSDMA, GSDMB, GSDMC, GSDMD, GSDME (DFNA5) and DFNB59 (Pejvakin). Members of the gasdermin family are expressed in a variety of cell types including epithelial cells and immune cells. GSDMA, GSDMB, GSDMC, GSDMD and GSDME have been suggested to act as tumour suppressors. Several current studies have revealed that GSDMD serves as a specific substrate of inflammatory caspases (caspase-1, -4, -5 and -11) and as an effector molecule for the lytic and highly inflammatory form of programmed cell death known as pyroptosis. Hence, GSDMD is an essential mediator of host defence against microbial infection and danger signals. The pore-forming activity of the N-terminal cleavage product causes cell swelling and lysis to prevent intracellular pathogens from replicating, and is required for the release of cytoplasmic content such as the inflammatory cytokine interleukin-1β (IL-1β) into the extracellular space to recruit and activate immune cells to the site of infection. GSDMD has an additional potential role as an antimicrobial by binding to cardiolipin (CL) and form pores on bacterial membranes. Mutation of GSDMD is associated with various genetic diseases and human cancers, including brain, breast, lung, urinary bladder, cervical, skin, oral cavity, pharynx, colon, liver, cecum, stomach, pancreatic, prostate, oesophageal, head and neck, hematologic, thyroid and uterine cancers.

背景文献

1. Shi H. et. al. GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury. Circ Res. 2021 Jul

2. Wang K. et. al. Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis. Cell. 2020 Mar

亚细胞定位

Cytosol, Inflammasome; Secreted, Cell membrane.

UNIPROT

SwissProt: P57764 Human

SwissProt: Q9D8T2 Mouse

别名

1810036L03Rik antibody

DF 5L antibody

DF5L antibody

DFNA 5L antibody

DFNA5L antibody

FKSG 10 antibody

FKSG10 antibody

FLJ12150 antibody

Gasdermin antibody

Gasdermin D antibody

展开

图片

  • Western blot analysis of GSDMD on different lysates with Mouse anti-GSDMD antibody (<a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>) at 1/500 dilution.<br /><br />Lane 1: PC-3 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: THP-1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601046" style="font-weight: bold;text-decoration: underline;">HA601046</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GSDMD on different lysates with Mouse anti-GSDMD antibody (HA601046) at 1/500 dilution.

    Lane 1: PC-3 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: THP-1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601046) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

同靶点 & 同通路的产品

metafield image

GSDMD Mouse Monoclonal Antibody [A8D8]

Application: WB

Reactivity: Human

Conjugate: unconjugated

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