FIP200 Mouse Monoclonal Antibody [A9A10]
Catalog# HA601107
FIP200 Mouse Monoclonal Antibody [A9A10]
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WB
-
Human
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Mouse
-
unconjugated
概述
产品名称
FIP200 Mouse Monoclonal Antibody [A9A10]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human RB1CC1 aa 651-800 / 1,594.
种属反应性
Human, Mouse
验证应用
WB
分子量
Predicted band size: 183 kDa
阳性对照
Hela cell lysate, MG-63 cell lysate, MCF-7 cell lysate, 293T cell lysate, K562 cell lysate, A431 cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate.
偶联
unconjugated
克隆号
A9A10
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members. FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation. FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration. FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation. FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy.
背景文献
1. Yeo SK et al. Role of FIP200 in inflammatory processes beyond its canonical autophagy function. Biochem Soc Trans. 2020 Aug
2. Wang L et al. FIP200 restricts RNA virus infection by facilitating RIG-I activation. Commun Biol. 2021 Jul
亚细胞定位
Nucleus, Cytoplasm, Cytoplasm, cytosol, Lysosome.
别名
FAK family kinase-interacting protein of 200 kDa antibody
FIP200 antibody
RB1-inducible coiled-coil protein 1 antibody
RB1CC1 antibody
RBCC1_HUMAN antibody
图片
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Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601107) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: MG-63 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: 293T cell lysate
Lane 5: K562 cell lysate
Lane 6: A431 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: HepG2 cell lysate
Lane 9: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 250/150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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