F4/80 Recombinant Antibody [PSH01-87] - Guinea pig IgG2 (Chimeric)
概述
产品名称
F4/80 Recombinant Antibody [PSH01-87] - Guinea pig IgG2 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse F4/80 aa 1-650 / 931.
种属反应性
Mouse
验证应用
WB, IHC-Fr
分子量
Predicted band size: 102 kDa
阳性对照
Mouse spleen tissue lysate.
偶联
unconjugated
克隆号
PSH01-87
反应性数据
| WB | IHC-Fr | |
|---|---|---|
| Mouse |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-Fr
-
1:200
靶点
功能
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
背景文献
1. Shin AE et al. F4/80(+)Ly6C(high) Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology. 2023 Apr
2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
亚细胞定位
Cell membrane.
UNIPROT
别名
ADGRE1 antibody
Adhesion G protein coupled receptor E1 antibody
Adhesion G protein-coupled receptor E1 antibody
AGRE1_HUMAN antibody
Cell surface glycoprotein EMR1 antibody
Cell surface glycoprotein F4/80 antibody
DD7A5 7 antibody
Egf like module containing mucin like hormone receptor like 1 antibody
Egf like module containing mucin like hormone receptor like sequence 1 antibody
EGF like module receptor 1 antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of F4/80 on different lysates with Guinea pig anti-F4/80 antibody (HA601567) at 1/2,000 dilution.
Lane 1: L-929 cell lysate (negative)
Lane 2: Mouse spleen tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 150 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601567) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: liver
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: spleen
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Integrated Single-Cell Profiling Reveals TL1A as a Biomarker and Driver of Type 2 Inflammation via Macrophage-Dependent Immunoregulation in Asthma
期刊: Research
DOI: 10.34133/research.1190
IF: 10.7
应用: WB
反应种属: Mouse
发表时间: 2026 Feb
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