F13A1 Mouse Monoclonal Antibody [16D3]
Catalog# EM1901-38
F13A1 Mouse Monoclonal Antibody [16D3]
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WB
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IHC-P
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FC
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Human
概述
产品名称
F13A1 Mouse Monoclonal Antibody [16D3]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human F13A1 aa 259-463 / 732.
种属反应性
Human
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 83 kDa
阳性对照
Human placenta tissue lysates, human placenta tissue, A549.
偶联
unconjugated
克隆号
16D3
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
靶点
功能
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and factors V, VIII, IX and X), which are involved in a blood coagulation cascade leading to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IXa, factor Xa, factor XIIa, or thrombin mediated proteolytic cleavage of Factor VII at Arg152-Ile153 generates Factor VIIa, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing two EGF-like domains. Coagulation factor XIII is a terminal effector in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of two A subunits and two B subunits. The A subunits have catalytic function, and the noncatalytic B subunits may serve as plasma carrier molecules.
背景文献
1. Thomas A. et. al. Coagulation factor XIIIA subunit missense mutations affect structure and function at the various steps of factor XIII action. Hum. Mutat. 37:1030-1041(2016).
序列相似性
Belongs to the transglutaminase superfamily. Transglutaminase family.
翻译后修饰
The activation peptide is released by thrombin.
亚细胞定位
Secreted, cytoplasm.
UNIPROT
别名
bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
Coagulation factor XIII A chain antibody
Coagulation factor XIII A1 polypeptide antibody
Coagulation factor XIII A1 subunit antibody
Coagulation factor XIII, A polypeptide antibody
Coagulation factor XIIIa antibody
F13A antibody
F13A_HUMAN antibody
F13a1 antibody
Factor XIIIA antibody
展开图片
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Western blot analysis of F13A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-F13A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-38, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of F13A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-38, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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