E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45]
Catalog# HA721362
E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45]
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WB
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Human
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HA750625
不含抗保成分
-
unconjugated
概述
产品名称
E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human E2F4 aa 21-80 / 413.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 44 kDa
阳性对照
293T cell lysate, Raji cell lysate, HeLa cell lysate, Jurkat cell lysate.
偶联
unconjugated
克隆号
PSH0-45
RRID
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
背景文献
1. Xiao W et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022 Nov
2. Wang Y et al. Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis. Protein Cell. 2022 Oct
亚细胞定位
Nucleus.
UNIPROT
别名
E2F 4 antibody
E2F transcription factor 4 antibody
E2F transcription factor 4 p107/p130 binding antibody
E2F-4 antibody
E2F4 antibody
E2F4_HUMAN antibody
p107/p130 binding protein antibody
Transcription factor E2F 4 antibody
Transcription factor E2F4 antibody
图片
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Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (HA721362) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: Raji cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 10 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721362) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
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