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Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody

RMB: 650

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Catalog# 016-303-001

Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

  • Flow Cyt

Reactivity

  • Species independent

Conjugation

  • unconjugated

概述

产品名称

Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody

抗体类型

Alpaca Nanobody

免疫原

DYKDDDDK tag fusion protein

产品特异性

DYKDDDDK tag sequence

种属反应性

Species independent

验证应用

WB, ICC/IF, ELISA, IP, Flow Cyt

偶联

unconjugated

产品特性

形态

Liquid

浓度

存放说明

Store at –20 ℃(Avoid freeze / thaw cycles), Stable for 12 months at -20 ℃.

存储缓冲液

10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol.

亚型

Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)

纯化方式

Recombinant Expression and Affinity purified.

应用稀释度

  • WB

  • 1:5,000-1:20,000

  • ELISA

  • 1:5,000-1:20,000

  • ICC/IF

  • 1:200-1:1,000

  • IP

  • 1-2µg/sample

  • Flow Cyt

  • 1µg for 10^6 cells

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

靶点

功能

Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.

背景文献

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