DDX39B Mouse Monoclonal Antibody [B2-A2]
Catalog# EM1706-69
DDX39B Mouse Monoclonal Antibody [B2-A2]
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WB
-
IHC-P
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Human
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Mouse
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unconjugated
概述
产品名称
DDX39B Mouse Monoclonal Antibody [B2-A2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Purified recombinant fragment of human DDX39B (AA: 1-250) expressed in E. Coli.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
49 kDa
阳性对照
Human DDX39B recombinant protein, DDX39B-hIgGFc transfected HEK293 cell lysate, HEK293, Jurkat, MCF-7, A431, NIH/3T3, K562, HepG2, human cervical cancer tissues.
偶联
unconjugated
克隆号
B2-A2
产品特性
形态
Liquid
浓度
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
靶点
功能
BAT1 (spliceosome RNA helicase BAT1), also known as DEAD-box protein UAP56 (56 kDa U2AF65-associated protein), HLA-B associated transcript-1 or ATP-dependent RNA helicase p47, is a member of the DECD subfamily of DEAD-box helicases. Important for mRNA splicing and nuclear export, BAT1 interacts with the mRNA export factor ALY and also functions as a splicing factor, mediating the first ATP-dependent step of spliceosome assembly. BAT1 associates with transcription elongation factor THO proteins, forming the TREX complex, and also interacts with splicing machinery to form the exon junction complex. Due to alternative splicing events, BAT1 exists in two isoforms. DDX39 (DEAD box protein 39), like BAT1, is a member of the DEAD-box family of helicases. Localized to the nucleus and expressed in lung, brain, kidney, splenn, thymus and salivary gland, DDX39 functions in a similar manner to BAT1 and is involved in pre-mRNA splicing and mRNA export out of the nucleus. DDX39 expression is upregulated in lung squamous cell carcinoma, suggesting a role for DDX39 in tumorigenesis.
背景文献
1. Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010).
亚细胞定位
Nucleus. Nucleus speckle
别名
0610030D10Rik antibody
4F2-LC6 antibody
56 kDa U2AF65-associated protein antibody
AI428441 antibody
ATP-dependent RNA helicase p47 antibody
B(0
+)-type amino acid transporter 1 antibody
BAT1 antibody
Bat1a antibody
D17H6S81E antibody
展开图片
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Western blot analysis of DDX39B on HEK293 (1) and DDX39B-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX39B antibody at 1/1,000 dilution.
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Western blot analysis of DDX39B on different cells lysates using anti-DDX39B antibody at 1/1,000 dilution.
Positive control: Line1: HEK293 Line2: Jurkat Line3: MCF-7 Line4: A431 Line5: NIH/3T3 Line6: Jurkat Line7: K562 Line8: HepG2 -
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-DDX39B antibody. Counter stained with hematoxylin.
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