Skip to content

Cyclin B1 Mouse Monoclonal Antibody [C2-F2]

RMB: 1500

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# M1508-1

Cyclin B1 Mouse Monoclonal Antibody [C2-F2]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Cyclin B1 Mouse Monoclonal Antibody [C2-F2]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide (KLH-coupled) within human Cyclin B1 aa 391-433.

种属反应性

Human

验证应用

WB, IF-Cell

分子量

Predicted band size: 48 kDa

阳性对照

K562 cell lysates, HepG2, LOVO.

偶联

unconjugated

克隆号

C2-F2

RRID

AB_3073117

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500

  • IF-Cell

  • 1:200-1:500

发表文章中的应用

WB 查看 1 篇文献如下
IF 查看 1 篇文献如下

发表文章中的种属

White goats 查看 1 篇文献如下

靶点

功能

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. The active complex phosphorylates and activates 13S condensin, which helps to condense chromosomes. Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. The nuclear envelope is a membranous structure containing large protein complexes supported by a network of nuclear lamins. Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. The destruction of the nuclear envelope is important because it allows the mitotic spindle to access the chromosomes.

背景文献

1. Cheng J et al. Knockout of cyclin B1 in granulosa cells causes female subfertility. Cell Cycle. 2022 Sep

2. Chen NP et al. CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry. Nat Cell Biol. 2022 May

序列相似性

Belongs to the cyclin family. Cyclin AB subfamily.

翻译后修饰

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

亚细胞定位

Cytoplasm, Nucleus, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: P14635 Human

别名

CCNB 1 antibody

CCNB antibody

ccnb1 antibody

CCNB1_HUMAN antibody

Cyclin B1 antibody

G2 mitotic specific cyclin B1 antibody

G2/mitotic-specific cyclin-B1 antibody

图片

  • Western blot analysis of Cyclin B1 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 58 kDa

    Western blot analysis of Cyclin B1 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

    Predicted band size: 48 kDa
    Observed band size: 58 kDa

  • ICC staining Cyclin B1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade;555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining Cyclin B1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

  • ICC staining Cyclin B1 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc&trade;555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

    ICC staining Cyclin B1 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IF

同靶点 & 同通路的产品

metafield image

Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP

Reactivity: Human

Conjugate: unconjugated

metafield image

Cyclin B1 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

请您留言

杭州华安生物技术有限公司 0571-88062880

您需要的服务
抗体咨询与购买
提交