Caspase-8 Mouse Monoclonal Antibody [6G1]
Catalog# RT1099
Caspase-8 Mouse Monoclonal Antibody [6G1]
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WB
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IP
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IF
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IHC-P
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Human
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unconjugated
概述
产品名称
Caspase-8 Mouse Monoclonal Antibody [6G1]
抗体类型
Mouse Monoclonal Antibody
免疫原
peptide
种属反应性
Human
验证应用
WB, IP, IF, IHC-P
分子量
55kDa
阳性对照
Hela, Jurkat, CCRF-CEM, MOLT-4
偶联
unconjugated
克隆号
6G1
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃
存储缓冲液
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:100-1:1,000
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IP
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1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
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IF
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1:50-500
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IHC-P
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1:50-500
靶点
功能
Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.
背景文献
1. Wang, O., et al. 2011. Anticancer activity of 2α, 3α, 19β, 23β-Tetrahydroxyurs-12-en-28-oic acid (THA), a novel triterpenoid isolated from Sinojackia sarcocarpa. PLoS ONE 6: e21130.
2. Cleveland, J.L., et al. 1995. Contenders in FAS-L/TNF death signaling. Cell 81: 479-482.
亚细胞定位
Cytoplasm
UNIPROT
别名
ALPS2B antibody
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
Apoptosis related cysteine peptidase antibody
Apoptotic cysteine protease antibody
Apoptotic protease Mch-5 antibody
Apoptotic protease Mch5 antibody
CAP 4 antibody
CAP4 antibody
CASP-8 antibody
CASP8 antibody
展开图片
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Western blot analysis of caspase-8 expression in Jurkat whole cell lysate.
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Western blot analysis of caspase-8 expression in CCRF-CEM (A), HeLa (B) and MOLT-4 (C) whole cell lysates.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Reduction of D2 receptors on microglia leads to ZBP1-mediated PANoptosis of mPFC in Parkinson's disease depression mice
期刊: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.114809
IF: 4.8
应用: WB,IF
反应种属: Mouse
发表时间: 2025 May
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LncRNA-MEG3 Mediated Diabetic Cerebral Ischemia–Reperfusion Injury-Induced Apoptosis via Modulating Interaction Between Annexin A2 and Akt in Mitochondria
期刊: CNS Neuroscience & Therapeutics
DOI: 10.1111/cns.70242
IF: 4.8
应用: WB
反应种属: Rat
发表时间: 2025 Feb
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Costunolide: Targeting Endothelial Cell PANoptosis to Mitigate Lung Injury in Acute Pancreatitis Mice
期刊: Phytomedicine
DOI: 10.1016/j.phymed.2025.156767
IF: 6.7
应用: WB
反应种属: Human,Mouse
发表时间: 2025 Apr
同靶点 & 同通路的产品
Caspase-8 Recombinant Rabbit Monoclonal Antibody [SD08-06]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human
Conjugate: unconjugated
Caspase-8 Recombinant Rabbit Monoclonal Antibody [PSH04-77]
Application: WB,IF-Cell,IHC-P,FC,IP
Reactivity: Human
Conjugate: unconjugated
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