Cardiac Troponin T Mouse Monoclonal Antibody [5-C7]
Catalog# M1302-1
Cardiac Troponin T Mouse Monoclonal Antibody [5-C7]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
概述
产品名称
Cardiac Troponin T Mouse Monoclonal Antibody [5-C7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human TNNT2 50-100 aa.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 36 kDa
阳性对照
Human heart tissue lysate, SK-Br-3, mouse heart tissue.
偶联
unconjugated
克隆号
5-C7
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:2,000-1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).Troponin T type 2 (cTnT,TNNT2) is a cardiac troponin T isoform expressed in the human heart, that is essential for calcium-regulated myofibrillar ATPase activity. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.
背景文献
1. Gerull B et al. A rapid protocol for cardiac troponin T gene mutation detection in familial hypertrophic cardiomyopathy. Hum Mutat 11:179-182 (1998).
2. Thierfelder L et al. Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere. Cell 77:701-712 (1994).
序列相似性
Belongs to the troponin T family.
组织特异性
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
翻译后修饰
Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
亚细胞定位
Cytosol.
别名
Cardiac muscle troponin T antibody
Cardiomyopathy dilated 1D (autosomal dominant) antibody
Cardiomyopathy hypertrophic 2 antibody
CMD1D antibody
CMH2 antibody
CMPD2 antibody
cTnT antibody
LVNC6 antibody
MGC3889 antibody
OTTHUMP00000033864 antibody
展开图片
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Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.
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ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
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Cardiac Troponin T Recombinant Rabbit Monoclonal Antibody [SC64-03] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
