Calpain 1 Mouse Monoclonal Antibody [2A2]
Catalog# EM1801-03
Calpain 1 Mouse Monoclonal Antibody [2A2]
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Calpain 1 Mouse Monoclonal Antibody [2A2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human Calpain 1 aa 1-714 / 714.
种属反应性
Human, Mouse, Rat
验证应用
IF-Cell, IHC-P, FC
分子量
Predicted band size: 82 kDa
阳性对照
Rat testis tissue, human spleen tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, A431, SiHa.
偶联
unconjugated
克隆号
2A2
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgM
纯化方式
Protein L affinity purified.
应用稀释度
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
Calpain 1 (large subunit), also designated µ-calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1 (large subunit), Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
背景文献
1. Sorimachi H et al. A novel member of the calcium-dependent cysteine protease family. Biol Chem Hoppe-Seyler 371:171-176 (1990).
2. Hsu C Y et al. Deimination of human filaggrin-2 promotes its proteolysis by Calpain 1 (large subunit). J Biol Chem 286:23222-23233 (2011).
序列相似性
Belongs to the peptidase C2 family.
组织特异性
Ubiquitous.
翻译后修饰
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
亚细胞定位
Cell membrane, Cytoplasm, Membrane.
别名
Ca2 activated neutral protease antibody
Calcium activated neutral proteinase antibody
Calcium activated neutral proteinase small subunit antibody
Calcium dependent protease small subunit 1 antibody
Calcium dependent protease small subunit antibody
Calcium-activated neutral proteinase 1 antibody
Calpain 1 antibody
Calpain 1 large subunit antibody
Calpain 1, (mu/I) large subunit antibody
Calpain mu type antibody
展开图片
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ICC staining Calpain 1(green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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