Cytokeratin 5 Mouse Monoclonal Antibody [6D-4D]
Catalog# EM1706-36
Cytokeratin 5 Mouse Monoclonal Antibody [6D-4D]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Monkey
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unconjugated
概述
产品名称
Cytokeratin 5 Mouse Monoclonal Antibody [6D-4D]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human, Mouse, Monkey
验证应用
WB, IHC-P, IF-Cell, FC
分子量
62 kDa
阳性对照
A431, 3T3-L1, COS7, HepG2, Hela, MCF-7, SK-Br-3, Lncap, human CK5 recombinant protein, human ovarian cancer tissue.
偶联
unconjugated
克隆号
6D-4D
产品特性
形态
Liquid
浓度
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-cell
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1:200-1:1000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).
背景文献
1. Leccia, F. et al. Cytometric and biochemical characterization of human breast cancer cells reveals heterogeneous myoepithelial phenotypes. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81: 960-72 (2012).
2. Lara, MF. et al. Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays. Hum. Gene Ther.. 23: 816-23 (2012).
亚细胞定位
Cytoplasm.
别名
58 kDa cytokeratin antibody
CK-5 antibody
CK5 antibody
Cytokeratin-5 antibody
Cytokeratin5 antibody
DDD antibody
DDD1 antibody
EBS2 antibody
epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
K2C5_HUMAN antibody
展开图片
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Western blot analysis of CK5 on different cells lysates using anti-CK5 antibody at 1/1,000 dilution.
Positive control:
Line1: A431
Line2: 3T3-L1
Line3: COS7
Line4: MCF-7
Line5: SK-Br-3
Line6: Hela
Line7: Lncap
Line8: HepG2 -
Western blot analysis of CK5 on human CK5 recombinant protein using anti-CK5 antibody at 1/1,000 dilution.
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Western blot analysis of CK5 on HEK293 (1) and CK5-hIgGFc transfected HEK293 (2) cell lysate using anti-CK5 antibody at 1/1,000 dilution.
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ICC staining CK5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- CK5 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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