CD64 Mouse Monoclonal Antibody [A7E4]
Catalog# HA601003
CD64 Mouse Monoclonal Antibody [A7E4]
-
WB
-
Human
概述
产品名称
CD64 Mouse Monoclonal Antibody [A7E4]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CD64 aa 1-200 / 374.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 43 kDa
阳性对照
THP-1 cell lysates, U937 cell lysates.
偶联
unconjugated
克隆号
A7E4
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
靶点
功能
CD64 (Cluster of Differentiation 64) is a type of integral membrane glycoprotein known as an Fc receptor that binds monomeric IgG-type antibodies with high affinity. It is more commonly known as Fc-gamma receptor 1 (FcγRI). After binding IgG, CD64 interacts with an accessory chain known as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. Structurally CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail.CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. There are three distinct (but highly similar) genes in humans for CD64 called FcγRIA (CD64A), FcγRIB (CD64B), and FcγRIC (CD64C) that are located on chromosome 1. These three genes produce six different mRNA transcripts; two from CD64A, three from CD64B, and one from CD64C; by alternate splicing of the genes.
背景文献
1. Patnaik R. et. al. Neutrophil CD64 a Diagnostic and Prognostic Marker of Sepsis in Adult Critically Ill Patients: A Brief Review. Indian J Crit Care Med. 2020 Dec
2. Walcheck B. et. al. iNK-CD64/16A cells: a promising approach for ADCC? Expert Opin Biol Ther. 2019 Dec
亚细胞定位
Cell membrane.
UNIPROT
别名
CD 64 antibody
CD64 antibody
CD64 antigen antibody
CD64A antibody
CD64b antibody
CD64c antibody
Fc fragment of IgG high affinity Ia receptor antibody
Fc fragment of IgG high affinity Ib receptor antibody
Fc fragment of IgG high affinity Ic receptor antibody
Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
展开图片
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Western blot analysis of CD64 on THP-1 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 70 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD64 on U937 cell lysates with Mouse anti-CD64 antibody (HA601003) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 70 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601003) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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