CD25 / IL-2 Receptor alpha Recombinant Mouse Monoclonal Antibody [PSH14-46]
Catalog# HA601486
CD25 / IL-2 Receptor alpha Recombinant Mouse Monoclonal Antibody [PSH14-46]
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FC
-
IHC-P
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IF-Tissue
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Human
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Mouse
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unconjugated
概述
产品名称
CD25 / IL-2 Receptor alpha Recombinant Mouse Monoclonal Antibody [PSH14-46]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CD25 aa 22-240 (Extracellular).
种属反应性
Human, Mouse
验证应用
FC, IHC-P, IF-Tissue
分子量
Predicted band size: 31 kDa
阳性对照
Human peripheral blood, mouse spleen tissue.
偶联
unconjugated
克隆号
PSH14-46
反应性数据
| FC | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
|
||
| Mouse |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
FC
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1:1,000 (For concentrate), 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood (For predilute)
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IHC-P
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1:1,000
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IF-Tissue
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1:1,000
靶点
功能
The interleukin-2 receptor alpha chain (also called Tac antigen, P55, and mainly CD25) is a protein involved in the assembly of the high-affinity interleukin-2 receptor, consisting of alpha (IL2RA), beta (IL2RB) and the common gamma chain (IL2RG). As the name indicates, this receptor interacts with interleukin-2, a pleiotropic cytokine which plays an important role in immune homeostasis.
背景文献
1. Pan Z et al. IL2RA(+)VSIG4(+) tumor-associated macrophage is a key subpopulation of the immunosuppressive microenvironment in anaplastic thyroid cancer. Biochim Biophys Acta Mol Basis Dis. 2023 Jan
2. Nguyen CH et al. IL2RA Promotes Aggressiveness and Stem Cell-Related Properties of Acute Myeloid Leukemia. Cancer Res. 2020 Oct
亚细胞定位
Membrane.
别名
Interleukin 2 receptor alpha chain antibody
CD25 antibody
CD25 antigen antibody
IDDM10 antibody
IL 2 receptor alpha subunit antibody
IL-2 receptor subunit alpha antibody
IL-2-RA antibody
IL-2R subunit alpha antibody
IL2 RA antibody
IL2 Receptor alpha antibody
展开图片
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Flow cytometric analysis of human peripheral blood cells labeling CD25 / IL-2 Receptor alpha (HA601486) and CD4 (HA600121F, APC).
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601486, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Mouse IgG Isotype Control (iFluor™ 488, 1/1,000). -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD25 / IL-2 Receptor alpha antibody (HA601486) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601486) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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