CD10 Rabbit Polyclonal Antibody
Catalog# 0805-4
CD10 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
CD10 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within Human CD10 aa 205-254 / 750.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 86 kDa
阳性对照
LNCaP cell lysate, Ramos cell lysate, Raji cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:50-1:200
靶点
功能
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
背景文献
1. Morisaki N et al. Neprilysin is identical to skin fibroblast elastase: its role in skin aging and UV responses. J Biol Chem 285:39819-39827 (2010).
2. Auer-Grumbach M et al. Rare variants in MME, encoding metalloprotease neprilysin, are linked to late-onset autosomal-dominant axonal polyneuropathies. Am J Hum Genet 99:607-623 (2016).
序列相似性
Belongs to the peptidase M13 family.
翻译后修饰
Myristoylation is a determinant of membrane targeting.; Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
亚细胞定位
Cell membrane.
别名
Atriopeptidase antibody
CALLA antibody
CD10 antibody
CD10 antigen antibody
Common acute lymphocytic leukemia antigen antibody
DKFZp686O16152 antibody
EC 3.4.24.11 antibody
Enkephalinase antibody
EPN antibody
Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (0805-4) at 1/2,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: Ramos cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07]
Application: WB,FC,IHC-P,IF-Cell,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点&同通路的产品
CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07]
Application: WB,FC,IHC-P,IF-Cell,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD10 Mouse Monoclonal Antibody [A1G5]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
CD10 Recombinant Rabbit Monoclonal Antibody [JE60-45]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
CD10 Mouse Monoclonal Antibody [A1G3]
Application: WB,IHC-P,FC,IF-Tissue
Reactivity: Human,Mouse
Conjugate: unconjugated
CD10 Mouse Monoclonal Antibody [A1G4]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
