C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
Catalog# M1001-1
C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
概述
产品名称
C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human C19orf35 aa 1-473 / 473.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 51 kDa
阳性对照
Recombinant protein, Hela, MCF-7, PC-3M, human tonsil tissue, human lung tissue, human uterus tissue, human breast tissue.
偶联
unconjugated
克隆号
3-B7-G3
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
靶点
功能
C19orf35 and CNRIP1 were specific to diffuse-type compared to the intestinal-type subgroups. Both revealed a similar level of methylation in CNM regardless of the histologic subtype of tumor while the methylation of the tumor tissue varied with the histologic type. C19orf35 was hypermethylated but CNRIP1 was hypomethylated in diffuse-type tumors compared to the CNM while it had an opposite trend in the tumors of the intestinal-type and mixed-type subgroups. Importantly, research suggest the possibility of utilizing these genes as specific markers for diffuse-type GCs. On the other hand, the function of C19orf35 is still unknown while frequent methylation of CNRIP1 has recently been reported in colorectal cancers and adenoma.
背景文献
1. Chong Y et al. DNA methylation status of a distinctively different subset of genes is associated with each histologic Lauren classification subtype in early gastric carcinogenesis. Oncol Rep 31(6):2535-44 (2014).
序列相似性
Belongs to the protein kinase superfamily.
亚细胞定位
Actin cytoskeleton.
UNIPROT
别名
C19orf35 antibody
Chromosome 19 open reading frame 35 antibody
CS035_HUMAN antibody
FLJ45778 antibody
Putative uncharacterized protein C19orf35 antibody
图片
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Western blot analysis of C19orf35 on recombinant protein using anti-C19orf35 antibody at 1/1,000 dilution.
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ICC staining C19orf35 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C19orf35 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C19orf35 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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