BTRC Mouse Monoclonal Antibody [D8-C5]
Catalog# EM1706-79
BTRC Mouse Monoclonal Antibody [D8-C5]
-
WB
-
IHC-P
-
FC
-
Human
-
unconjugated
概述
产品名称
BTRC Mouse Monoclonal Antibody [D8-C5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Purified recombinant fragment of human BTRC (AA: 24-151) expressed in E. Coli.
种属反应性
Human
验证应用
WB, IHC-P, FC
分子量
69 kDa
阳性对照
Human BTRC recombinant protein, BTRC-hIgGFc transfected HEK293 cell lysate, Ramos, MCF-7, K562 cell lysates, human esophageal cancer tissue, human rectum cancer tissue, Hela.
偶联
unconjugated
克隆号
D8-C5
产品特性
形态
Liquid
浓度
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,000
-
FC
-
1:200-1:400
靶点
功能
b-tranducin repeats containing protein (b-TrCP), also designated E3RSIkB or FWD1, and HOS (homologous to Slimb) are F-box proteins that function as substrate recognition subunits of ubiquitin ligases. HOS and b-TrCP differ in their amino-terminal regions, but exhibit high homology within the F-box and WD40 repeat-containing regions. b-TrCP mediates ubiquitin/proteasome-dependent degradation of CD4 and ubiquitination of various proteins including IkB and b-catenin. HOS has also been shown to regulate the degradation of IkB and b-catenin in a similar manner.
背景文献
1. Schmidt ML et al. Ras regulates SCF(β-TrCP) protein activity and specificity via its effector protein NORE1A. J Biol Chem 289(45):31102-10 (2014).
2. Huo ZH et al. Roles of functional NFKB1 and β-TrCP insertion/deletion polymorphisms in mRNA expression and epithelial ovarian cancer susceptibility. Genet Mol Res 12(3):3435-43 (2013).
亚细胞定位
Cytoplasm. Nucleus
UNIPROT
别名
Beta transducin repeat containing E3 ubiquitin protein ligase antibody
beta TrCP1 antibody
beta-transducin repeat containing antibody
BETA-TRCP antibody
betaTrCP antibody
btrC antibody
bTrCP antibody
bTrCP1 antibody
E3RSIkappaB antibody
F box and WD repeat protein 1B antibody
展开图片
-
Western blot analysis of BTRC on human BTRC recombinant protein using anti-BTRC antibody at 1/1,000 dilution.
-
Western blot analysis of BTRC on HEK293 (1) and BTRC-hIgGFc transfected HEK293 (2) cell lysate using anti-BTRC antibody at 1/1,000 dilution.
-
Western blot analysis of BTRC on different cell lysate using anti-BTRC antibody at 1/1,000 dilution.
Positive control: Line1: Ramos Line2: MCF-7 Line3: K562 -
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-BTRC antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-BTRC antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
浙公网安备 33019202000643号