Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00]
概述
产品名称
Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Axin1 aa 1-400 / 862.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 95.6 kDa
阳性对照
SW480 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Huh7 cell lysate, human colon cancer tissue.
偶联
unconjugated
克隆号
PSH03-00
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:500-1:2,000
靶点
功能
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin . Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling . In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) . Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice . Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop .
背景文献
1. Luo W, Lin SC. Axin: a master scaffold for multiple signaling pathways. Neurosignals. 2004 May-Jun;13(3):99-113.
2. Salahshor S, Woodgett JR. The links between axin and carcinogenesis. J Clin Pathol. 2005 Mar;58(3):225-36.
3. Mallick A, Gupta BP. AXIN-AMPK signaling: Implications for healthy aging. F1000Res. 2021 Dec 8;10:1259.
亚细胞定位
Cytoplasm. Nucleus. Membrane. Cell membrane.
UNIPROT
别名
AI316800 antibody
AXIN antibody
Axin 1 antibody
Axin-1 antibody
axin1 antibody
AXIN1_HUMAN antibody
Axis inhibition protein 1 antibody
Axis Inhibitor 1 antibody
Fu antibody
fused antibody
展开图片
-
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
Lane 1: SW480 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: 293T cell lysate (10 µg/Lane)
Lane 4: K-562 cell lysate (10 µg/Lane)
Lane 5: Huh7 cell lysate (10 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Axin1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 140 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721919) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
C-Arg9-APCR3-VHL promotes β-catenin degradation via the VHL-mediated ubiquitin–proteasome system in APC-mutant colorectal cancer
期刊: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.146678
IF: 8.5
应用: WB
反应种属: Human
发表时间: 2025 Aug
-
DCI improves diabetic encephalopathy by modulating the BDNF/NF-κB/GSK-3β pathway
期刊: Experimental Neurology
DOI: 10.1016/j.expneurol.2025.115236
IF: 4.6
应用: WB
反应种属: Human
发表时间: 2025 Apr
-
Smoking-induced CCNA2 expression promotes lung adenocarcinoma tumorigenesis by boosting AT2/AT2-like cell differentiation
期刊: Cancer Letters
DOI:
IF: 9.7
应用: WB
反应种属: Human
发表时间: 2024 May
同靶点 & 同通路的产品
浙公网安备 33019202000643号