Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08] - BSA and Azide free
Catalog# HA750195
Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08] - BSA and Azide free
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
ET1609-77
含抗保成分
-
unconjugated
概述
产品名称
Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ACC1 aa 382-427 / 2346.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 266 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A549 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human kidney tissue, mouse placenta tissue.
偶联
unconjugated
克隆号
ST53-08
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:50-1:200
靶点
功能
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the enzyme’s short-term regulatory mechanism. The gene encoding ACCα maps to human chromosome 17 and encodes a form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of the ACCα, except for an additional peptide of about 150 amino acids at the N-terminus.
背景文献
1. Tiwari M et al. Loss of Caspase-2-dependent Apoptosis Induces Autophagy after Mitochondrial Oxidative Stress in Primary Cultures of Young Adult Cortical Neurons. J Biol Chem 286:8493-506 (2011).
2. Chang NW et al. Fenofibrate exhibits a high potential to suppress the formation of squamous cell carcinoma in an oral-specific 4-nitroquinoline 1-oxide/arecoline mouse model. Biochim Biophys Acta 1812:558-64 (2011).
组织特异性
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
翻译后修饰
Phosphorylation on Ser-1263 is required for interaction with BRCA1.; Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.; The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
亚细胞定位
Cytoplasm.
别名
ACAC antibody
ACACA antibody
ACACA_HUMAN antibody
ACC alpha antibody
ACC antibody
ACC-alpha antibody
ACC1 antibody
ACCA antibody
Acetyl CoA carboxylase 1 antibody
Acetyl CoA carboxylase alpha antibody
展开图片
-
Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (HA750195) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Predicted band size: 266 kDa
Observed band size: 250/266 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (HA750195) at 1/1,000 dilution.
Lane 1: HepG2-si NT cell lysate
Lane 2: HepG2-si ACC1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 266 kDa
Observed band size: 250/266 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (HA750195) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750195) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750195, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
浙公网安备 33019202000643号