ATRX Mouse Monoclonal Antibody [2-E7]
Catalog# M1311-2
ATRX Mouse Monoclonal Antibody [2-E7]
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
unconjugated
概述
产品名称
ATRX Mouse Monoclonal Antibody [2-E7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within mouse ATRX aa 2,236-2,416 / 2,476.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 283 kDa
阳性对照
SH-SY5Y cell lysate, NIH/3T3, mouse kidney tissue.
偶联
unconjugated
克隆号
2-E7
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:100
-
IHC-P
-
1:200-1:1,000
靶点
功能
Transcriptional regulator ATRX contains an ATPase / helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations of the ATRX gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes.
背景文献
1. Yuan K et al. Mutant ATRX: pathogenesis of ATRX syndrome and cancer. Front Mol Biosci. 2024 Oct
2. Aguilera P et al. ATRX, a guardian of chromatin. Trends Genet. 2023 Jun
序列相似性
Belongs to the SNF2/RAD54 helicase family.
翻译后修饰
Citrullinated by PADI4.
亚细胞定位
Nucleus, Chromosome, telomere, PML body.
别名
Alpha thalassemia/mental retardation syndrome X linked homolog antibody
ATP dependent helicase ATRX antibody
ATP-dependent helicase ATRX antibody
ATR2 antibody
Atrx antibody
ATRX_HUMAN antibody
DNA dependent ATPase and helicase antibody
Helicase 2, X linked antibody
MGC2094 antibody
MRXHF1 antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of ATRX on different lysates with Mouse anti-ATRX antibody (M1311-2) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: U-2 OS cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 283 kDa
Observed band size: 400 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1311-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling ATRX with Mouse anti-ATRX antibody (M1311-2) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATRX antibody (M1311-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-ATRX antibody (M1311-2) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1311-2) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号