14-3-3 alpha+beta Rabbit Polyclonal Antibody
Catalog# 0407-5
14-3-3 alpha+beta Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
14-3-3 alpha+beta Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within mouse 14-3-3 beta / alpha aa 197-246 / 246.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 28 kDa
阳性对照
Hela cell lysate, human brain tissue lysate, mouse brain tissue lysate, N2A.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:5,000
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IF-Cell
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1:200
靶点
功能
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol. The 14-3-3 proteins are also Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. They bind to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
背景文献
1. Carninci P., Kasukawa T., et al. Science 309:1559-1563(2005).
2. Sacksteder C.A., Qian W.-J.,et al. Biochemistry 45:8009-8022(2006).
3. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617–47 (2000).
序列相似性
Belongs to the 14-3-3 family.
翻译后修饰
Isoform alpha differs from isoform beta in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.; Isoform Short contains a N-acetylmethionine at position 1.
亚细胞定位
Cytoplasm, Melanosome.
别名
14 3 3 alpha antibody
14 3 3 protein beta/alpha antibody
14-3-3 protein beta/alpha antibody
1433B_HUMAN antibody
Brain protein 14 3 3 beta isoform antibody
GW128 antibody
HS 1 antibody
KCIP-1 antibody
KCIP1 antibody
N-terminally processed antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of 14-3-3 alpha+beta on different lysates with Rabbit anti-14-3-3 alpha+beta antibody (0407-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-14-3-3 alpha+beta KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.
Lane 1: Hela cell lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse brain tissue lysate
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ICC staining 14-3-3 b/a in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
14-3-3 alpha+beta Recombinant Rabbit Monoclonal Antibody [SD0837]
Application: WB,IF-Cell,IHC-P,FC,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点 & 同通路的产品
14-3-3 alpha+beta Recombinant Rabbit Monoclonal Antibody [SD0837]
Application: WB,IF-Cell,IHC-P,FC,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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