RON Recombinant Rabbit Monoclonal Antibody [SD2006]
Catalog# ET1612-80
RON Recombinant Rabbit Monoclonal Antibody [SD2006]
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WB
-
IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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unconjugated
概述
产品名称
RON Recombinant Rabbit Monoclonal Antibody [SD2006]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human RON aa 11-60 / 1,400.
种属反应性
Human
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 152 kDa
阳性对照
AGS cell lysate, A549 cell lysate, A549, AGS, A431, human lung cancer tissue, human breast cancer tissue.
偶联
unconjugated
克隆号
SD2006
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50
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IHC-P
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1:50
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IP
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Use at an assay dependent concentration.
靶点
功能
Receptor protein tyrosine kinases (PTKs) have been classified into different subclasses on the basis of sequence similarity and distinct structural characteristics. The c-Met encoded receptor represents the initial member of one class of receptors characterized by a heterodimeric structure and a cysteine-rich extracellular domain. Ron, also designated macrophage-stimulating protein receptor (MSP receptor), p185-Ron, CD136 antigen or PTK8 represents a second member of this receptor class. The intracellular PTK domains of Ron and Met are highly similar (63% sequence identity) while the extracellular domains are less related (25% sequence identity) and both are rich in cysteine residues. Mature Ron receptor is comprised of a disulfide-linked heterodimer formed from an alpha chain (Ron α) and a beta chain (Ron β). Proteolytic processing results in the separation of the N-terminal Ron α and C-terminal Ron β subunits.
背景文献
1. Wang Q et al. RON confers lapatinib resistance in HER2-positive breast cancer cells. Cancer Lett 340:43-50 (2013).
2. Seiwert, TY. et al. The MET receptor tyrosine kinase is a potential novel therapeutic target for head and neck squamous cell carcinoma. Cancer Res.. 69: 3021-3031(2009).
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
组织特异性
Expressed in colon, skin, lung and bone marrow.
翻译后修饰
Proteolytic processing yields the two subunits.; Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.; Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.
亚细胞定位
Membrane.
UNIPROT
别名
c met related tyrosine kinase antibody
CD136 antibody
CD136 antigen antibody
CDw136 antibody
Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
Macrophage stimulating 1 receptor antibody
Macrophage stimulating protein receptor alpha chain antibody
MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
Macrophage stimulating protein receptor beta chain antibody
Macrophage-Stimulating 1 Receptor (MST1R) antibody
展开图片
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Western blot analysis of RON on different lysates with Rabbit anti-RON antibody (ET1612-80) at 1/1,000 dilution.
Lane 1: AGS cell lysate
Lane 2: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 152 kDa
Observed band size: 152 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-80) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of RON in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of RON in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of RON in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-RON antibody (ET1612-80) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-80) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-RON antibody (ET1612-80) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-80) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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