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FITC Conjugated Goat anti-Rabbit IgG polyclonal Antibody

RMB: 300

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Catalog# HA1004

FITC Conjugated Goat anti-Rabbit IgG polyclonal Antibody

Application

  • IF-Cell

Reactivity

  • Rabbit

Conjugation

  • FITC

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

FITC Conjugated Goat anti-Rabbit IgG polyclonal Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Rabbit IgG (H+L).

种属反应性

Rabbit

验证应用

IF-Cell

偶联

FITC

产品特性

形态

Liquid

浓度

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • Immunocytochemistry

  • 1:100 – 1:500

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium.

背景文献

暂无

图片

  • ICC analysis of rabbit anti-Cytokeratin19 antibody (<a href="/products/ET1601-6" style="font-weight: bold;text-decoration: underline;">ET1601-6</a>,1/100) with AGS Cell lysates . Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Goat anti-rabbit IgG-FITC was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC analysis of rabbit anti-Cytokeratin19 antibody (ET1601-6,1/100) with AGS Cell lysates . Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Goat anti-rabbit IgG-FITC was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Goat anti-Rabbit IgG in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (PBS) for 1 hour at room temperature, washed with PBS. Goat anti-rabbit IgG-FITC was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Goat anti-Rabbit IgG in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (PBS) for 1 hour at room temperature, washed with PBS. Goat anti-rabbit IgG-FITC was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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