NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)
Catalog# HA601481
NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)
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IHC-Fr
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WB
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Mouse
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Rat
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HA610315
不含抗保成分
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unconjugated
概述
产品名称
NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within human NeuN aa 20-60.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, WB
分子量
Predicted band size: 34 kDa
阳性对照
Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
SR45-07
反应性数据
| IHC-Fr | WB | |
|---|---|---|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:200
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WB
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1:10,000
靶点
功能
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
背景文献
1. Santamaría G et al. NeuN distribution in brain structures of normal and Zika-infected suckling mice. J Mol Histol. 2023 Jun
2. Luijerink L et al. Immunostaining for NeuN Does Not Show all Mature and Healthy Neurons in the Human and Pig Brain: Focus on the Hippocampus. Appl Immunohistochem Mol Morphol. 2021 Jul
亚细胞定位
Nucleus, Cytoplasm.
别名
FLJ56884 antibody
FLJ58356 antibody
Fox-1 homolog C antibody
fox1 homolog C antibody
Fox3 antibody
FOX3NeuN antibody
hexaribonucleotide binding protein 3 antibody
HRNBP3 antibody
NEUN antibody
neuronal nuclei antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
☑ Relative expression (RE)
Western blot analysis of NeuN on different lysates with Guinea pig anti-NeuN antibody (HA601481) at 1/10,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: C2C12 cell lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 45/50 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601481) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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