Biotin Conjugated Human YKL-40 Recombinant Rabbit Monoclonal Antibody [PSH13-09] - Detector
概述
产品名称
Biotin Conjugated Human YKL-40 Recombinant Rabbit Monoclonal Antibody [PSH13-09] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human YKL-40 aa 22-383 (HA211030).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human YKL-40 protein (HA211030).
偶联
Biotin
克隆号
PSH13-09
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-08] to Human YKL-40 antibody (Capture) (HA725088) and Recombinant Human YKL-40 protein (HA211030) as the standard. The reference range value is 31.3-4,000 pg/ml.
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ELISA
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Use at an assay dependent concentration.
靶点
功能
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
背景文献
1. Connolly K et al. Potential role of chitinase-3-like protein 1 (CHI3L1/YKL-40) in neurodegeneration and Alzheimer\'s disease. Alzheimers Dement. 2023 Jan
2. Yu JE et al. Significance of chitinase-3-like protein 1 in the pathogenesis of inflammatory diseases and cancer. Exp Mol Med. 2024 Feb
亚细胞定位
Secreted, extracellular space, Cytoplasm, perinuclear region, Endoplasmic reticulum.
UNIPROT
别名
39 kDa synovial protein antibody
ASRT7 antibody
Cartilage glycoprotein 39 antibody
CGP-39 antibody
CGP39 antibody
CH3L1_HUMAN antibody
CHI3L1 antibody
chitinase 3 like 1 (cartilage glycoprotein 39) antibody
chitinase 3 like 1 antibody
Chitinase 3 like protein 1 precursor antibody
展开图片
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![Sandwich ELISA analysis of Human YKL-40 matched pair antibodies<br /><br />Capture: <a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>, Human YKL-40 Rabbit mAb [PSH13-08]<br />Detector: <a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Human YKL-40 Rabbit mAb [PSH13-09]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (<a href="/products/HA211030" style="font-weight: bold;text-decoration: underline;">HA211030</a>) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725090B_1.png?v=20250404172429)
Sandwich ELISA analysis of Human YKL-40 matched pair antibodies
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725088" style="font-weight: bold;text-decoration: underline;">HA725088</a>, Human YKL-40 Rabbit mAb [PSH13-08]<br />Detector: <a href="/products/HA725089" style="font-weight: bold;text-decoration: underline;">HA725089</a>, Human YKL-40 Rabbit mAb [PSH13-09]<br /><br />Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.](https://storage.huabio.cn/huabio/productImg/HA725090B_2.png?v=20250404172438)
Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load.
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.
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