FAB1A Rabbit Polyclonal Antibody
Catalog# HA500403
FAB1A Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Arabidopsis thaliana
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unconjugated
概述
产品名称
FAB1A Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within Arabidopsis thaliana FAB1A aa 100-200 / 1757.
种属反应性
Arabidopsis thaliana
验证应用
IF-Tissue, IHC-P
分子量
196 kDa
阳性对照
A. thaliana tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
靶点
功能
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate (By similarity). Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.
背景文献
1. Hirano T. et. al. Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis. Plant Physiol. 155:797-807(2011).
2. Hirano T. et. al. Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters. Plant Signal. Behav. 6:583-585(2011).
亚细胞定位
Endosome membrane.
UNIPROT
别名
1-phosphatidylinositol-3-phosphate 5-kinase FAB1A
Phosphatidylinositol 3-phosphate 5-kinase
FYVE finger-containing phosphoinositide kinase
PIKfyve
Phosphatidylinositol 3-phosphate 5-kinase type III
PIPkin-III
Type III PIP kinase
Protein FORMS APLOID AND BINUCLEATE CELLS 1A
FAB1A
At4g33240
展开图片
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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-FAB1A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with HA500403 at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-FAB1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500403, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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