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GRP94 Mouse Monoclonal Antibody [F1-D10-G3]

RMB: 900 特惠 1500

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Catalog# M1701-12

GRP94 Mouse Monoclonal Antibody [F1-D10-G3]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GRP94 Mouse Monoclonal Antibody [F1-D10-G3]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within Human GRP94 aa 201-400 / 803.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

分子量

Predicted band size: 94 kDa

阳性对照

HepG2 cell lysate, HeLa cell lysate, human brain tissue lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat placenta tissue lysate, rat brain tissue lysate, human liver cancer tissue, human kidney tissue.

偶联

unconjugated

克隆号

F1-D10-G3

RRID

AB_3073167

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgM

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:10,000

  • IHC-P

  • 1:50-1:200

发表文章中的应用

WB 查看 2 篇文献如下
IF 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下
Mouse 查看 1 篇文献如下

靶点

功能

The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and Proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.

背景文献

1. Gallagher CM et al. Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6a branch. Elife 5.pii: e11878 (2016).

2. Plate L et al. Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation. Elife 5.pii: e15550 (2016).

序列相似性

Belongs to the heat shock protein 90 family.

亚细胞定位

Endoplasmic reticulum lumen, melanosome.

UNIPROT

SwissProt: P14625 Human

SwissProt: P08113 Mouse

SwissProt: Q66HD0 Rat

别名

94 kDa glucose regulated protein antibody

94 kDa glucose-regulated protein antibody

ECGP antibody

Endoplasmin antibody

Endothelial cell (HBMEC) glycoprotein antibody

ENPL_HUMAN antibody

Glucose regulated protein 94kDa antibody

gp96 antibody

gp96 homolog antibody

GRP 94 antibody

展开

图片

  • Western blot analysis of GRP94 on different lysates with Mouse anti-GRP94 antibody (<a href="/products/M1701-12" style="font-weight: bold;text-decoration: underline;">M1701-12</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: Human brain tissue lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (10 µg/Lane)<br />Lane 5: Mouse placenta tissue lysate (20 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 7: PC-12 cell lysate (10 µg/Lane)<br />Lane 8: Rat placenta tissue lysate (20 µg/Lane)<br />Lane 9: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 94 kDa<br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1701-12" style="font-weight: bold;text-decoration: underline;">M1701-12</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GRP94 on different lysates with Mouse anti-GRP94 antibody (M1701-12) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: Human brain tissue lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
    Lane 5: Mouse placenta tissue lysate (20 µg/Lane)
    Lane 6: Mouse brain tissue lysate (20 µg/Lane)
    Lane 7: PC-12 cell lysate (10 µg/Lane)
    Lane 8: Rat placenta tissue lysate (20 µg/Lane)
    Lane 9: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 94 kDa

    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP94 antibody (<a href="/products/M1701-12" style="font-weight: bold;text-decoration: underline;">M1701-12</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1701-12" style="font-weight: bold;text-decoration: underline;">M1701-12</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP94 antibody (M1701-12) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  1. WB
  2. IF
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