GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]
Catalog# HA601279
GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]
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WB
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Human
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Mouse
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Rat
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HA610154
不含抗保成分
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unconjugated
概述
产品名称
GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human GOT1 aa 16-211 / 413.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 46 kDa
阳性对照
HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, Caco-2 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse heart tissue lysate, rat liver tissue lysate, rat heart tissue lysate.
偶联
unconjugated
克隆号
A2D6-R
RRID
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
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| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
背景文献
1. Abrego J. et. al. GOT1-mediated anaplerotic glutamine metabolism regulates chronic acidosis stress in pancreatic cancer cells. Cancer Lett. 2017 Aug 1;400:37-46.
2. Hong C. et. al. Inhibition of GOT1 sensitizes colorectal cancer cells to 5-fluorouracil. Cancer Chemother Pharmacol. 2017 Apr;79(4):835-840.
亚细胞定位
Cytoplasm.
别名
AATC_HUMAN antibody
Aspartate aminotransferase 1 antibody
Aspartate aminotransferase antibody
Aspartate aminotransferase cytoplasmic antibody
Aspartate aminotransferase cytosolic antibody
ASTQTL1 antibody
cAspAT antibody
cCAT antibody
cysteine aminotransferase, cytoplasmic antibody
cysteine transaminase, cytoplasmic antibody
展开图片
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Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse heart tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Rat heart tissue lysate (40 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 38 kDa
Exposure time: 1 minute 41 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GOT1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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