KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]
Catalog# HA723455
KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]
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WB
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Human
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Mouse
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Rat
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HA751444
不含抗保成分
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unconjugated
概述
产品名称
KCNA5 / KV1-5 Recombinant Rabbit Monoclonal Antibody [PSH12-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within rat KCNA5 aa 249-298 / 602.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 67 kDa
阳性对照
PANC-1 cell lysate, Mouse hippocampus tissue lysate, Mouse heart tissue lysate, Rat hippocampus tissue lysate, Rat heart tissue lysate.
偶联
unconjugated
克隆号
PSH12-30
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000
靶点
功能
KCNA5 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. It forms tetrameric, potassium-selective channels, allowing potassium ions to pass according to their electrochemical gradient. The channel alternates between open and closed states in response to changes in membrane voltage, contributing to the regulation of electrical activity in cells. KCNA5 can form functional homotetrameric channels or heterotetrameric channels with other KCNA family members, such as KCNA1, KCNA2, KCNA4, and KCNA5, which influence channel properties. This channel is critical for normal electrical conduction, including the formation of the ventricular conduction system and action potential configuration. It may also play a role in regulating insulin secretion in pancreatic islets. KCNA5 is associated with delayed rectifier potassium currents, which help restore the resting membrane potential after depolarization, particularly in beta cells. Defects in KCNA5 are linked to familial atrial fibrillation (ATFB7), a condition characterized by irregular heart rhythms that can lead to stroke or heart failure. Additionally, KCNA5 is implicated in pulmonary vascular function, where it helps set the resting membrane potential and participates in hypoxic pulmonary vasoconstriction.
背景文献
1. Williams CP, et al. Modulation of the human Kv1.5 channel by protein kinase C activation: role of the Kvbeta1.2 subunit. J Pharmacol Exp Ther. 2002 Aug.
2. Olson TM, et al. Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation. Hum Mol Genet. 2006 Jul.
亚细胞定位
Cell membrane.
别名
ATFB7 antibody
cardiac potassium channel antibody
HCK1 antibody
HK2 antibody
HPCN1 antibody
insulinoma and islet potassium channel antibody
KCNA5 antibody
KCNA5_HUMAN antibody
KV1.5 antibody
PCN1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of KCNA5 / KV1-5 on different lysates with Rabbit anti-KCNA5 / KV1-5 antibody (HA723455) at 1/1,000 dilution.
Lane 1: PANC-1 cell lysate(no heat) (15 µg/Lane)
Lane 2: Mouse hippocampus tissue lysate (no heat) (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)
Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)
Lane 7: Rat heart tissue lysate (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 1 minute 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723455) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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