Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

RMB: 1600 特惠 1600

产品规格

50μl

100μl

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Catalog# HA600091

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

Application

WB
IF-Cell

Reactivity

Human

概述

产品名称

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

种属反应性

Human

验证应用

WB, IF-Cell

分子量

Predicted band size: 15 kDa

阳性对照

Hela treated with TSA cell lysate, Hela, TSA treated Hela.

偶联

unconjugated

克隆号

A7B6

RRID

AB_3071707

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein G affinity purified.

应用稀释度

WB
1:2,000

IF-Cell
1:200

靶点

功能

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

背景文献

1. Benard A et al. Nuclear expression of histone deacetylases and their histone modifications predicts clinical outcome in colorectal cancer. Histopathology 66:270-82 (2015).

2. Zhang J et al. SOX4 inhibits GBM cell growth and induces G0/G1 cell cycle arrest through Akt-p53 axis. BMC Neurol 14:207 (2014).

亚细胞定位

Nucleus, Chromosome.

UNIPROT #

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q6NXT2 Human

SwissProt: Q71DI3 Human

别名

H3.3A antibody

HIST1 cluster, H3E antibody

H3 histone family, member A antibody

H3.1 antibody

H3/l antibody

H3F3 antibody

H3FF antibody

H3FJ antibody

H3FL antibody

Histone gene cluster 1, H3 histone family, member E antibody

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图片

☑ Cell treatment (CT)

Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/2,000 dilution.

Lane 1: Hela cell lysate
Lane 2: Hela treated with TSA cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 14 kDa

Exposure time: 30 seconds;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of TSA treated Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Histone H3 (acetyl K18) Mouse Monoclonal Antibody [A7B6]

RMB: 1600 特惠 1600

产品规格

联系我们

概述

产品名称

抗体类型

免疫原

种属反应性

验证应用

分子量

阳性对照

偶联

克隆号

RRID

产品特性

形态

浓度

存放说明

存储缓冲液

亚型

纯化方式

应用稀释度

靶点

功能

背景文献

亚细胞定位

UNIPROT #

别名

图片

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