TGF beta 1 Rabbit Polyclonal Antibody
Catalog# HA500496
TGF beta 1 Rabbit Polyclonal Antibody
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WB
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Human
概述
产品名称
TGF beta 1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human TGF beta 1 aa 20-210.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 44 kDa
阳性对照
PC-3 cell lysate, A549 cell lysate, U87 cell lysate, Hela cell lysate, K562 cell lysate.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500
发表文章中的应用
发表文章中的种属
| Rabbit | See 1 publications below |
靶点
功能
Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
背景文献
1. Robertson I.B., et al. Regulation of the bioavailability of TGF-beta and TGF-beta-related proteins. Cold Spring Harb. Perspect. Biol. 8:0-0(2016).
2. Miyazono K., et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. EMBO J. 10:1091-1101(1991) .
亚细胞定位
Extracellular matrix, Secreted.
UNIPROT #
别名
Cartilage-inducing factor antibody
CED antibody
Differentiation inhibiting factor antibody
DPD1 antibody
LAP antibody
Latency-associated peptide antibody
Prepro transforming growth factor beta 1 antibody
TGF beta 1 antibody
TGF beta antibody
TGF beta 1 protein antibody
展开图片
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Western blot analysis of TGF beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500496, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: U87 cell lysate
Lane 4: Hela cell lysate
Lane 5: K562 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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PEEP application during mechanical ventilation contributes to fibrosis in the diaphragm
Author: Qian, X., Jiang, Y., Jia, J., Shen, W., Ding, Y., He, Y., Xu, P., Pan, Q., Xu, Y., & Ge, H.
PMID: 36782202
应用: WB
反应种属: Rabbit
发表时间: 2023 Feb
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Citation
Alternative Products
TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点&同通路的产品
TGF beta 1 Mouse Monoclonal Antibody [B2-F6]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated