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Beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

RMB: 1500

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Catalog# ET1608-45

Beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within C-terminal human beta 2 Microglobulin.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell

分子量

Predicted band size: 14 kDa

阳性对照

Mouse spleen tissue lysate, mouse liver tissue lysate, rat spleen tissue lysate, rat liver tissue lysate, Raji cell lysate, U937 cell lysate, Hela, A431.

偶联

unconjugated

克隆号

SU35-05

RRID

AB_3069806

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

发表文章中的应用

WB See 2 publications below

发表文章中的种属

Human See 2 publications below
Mouse See 1 publications below

靶点

功能

β2 microglobulin also known as B2M is a component of MHC class I molecules, MHC class I molecules have α1, α2, and α3 proteins which are present on all nucleated cells (excludes red blood cells). In humans, the β2 microglobulin protein is encoded by the B2M gene. β2 microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 (5 genes in humans), MR1, the neonatal Fc receptor (FcRn), and Qa-1 (a form of alloantigen). Nevertheless, the β2 microglobulin gene is outside of the MHC (HLA) locus, on a different chromosome. An additional function is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the basolateral membrane of enterocytes and cell membrane of macrophages for degradation resulting in decreased iron uptake from food and decreased iron release from recycled red blood cells in the MPS (mononuclear phagocyte system) respectively. Loss of this function causes iron excess and hemochromatosis. In cytomegalovirus infection, a viral protein binds to β2 microglobulin, preventing assembly of MHC class I molecules and their transport to the plasma membrane.

背景文献

1. Yousef H et al. Systemic attenuation of the TGF- pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal. Oncotarget 6:11959-78 (2015).

2. Sabnis H et al. Capillary nano-immunoassay for Akt 1/2/3 and 4EBP1 phosphorylation in acute myeloid leukemia. J Transl Med 12:166 (2014).

序列相似性

Belongs to the beta-2-microglobulin family.

翻译后修饰

Glycation of Ile-21 is observed in long-term hemodialysis patients.

亚细胞定位

Secreted.

UNIPROT #

SwissProt: P61769 Human

SwissProt: P01887 Mouse

SwissProt: P07151 Rat

别名

B2M antibody

B2MG_HUMAN antibody

Beta 2 microglobin antibody

Beta 2 microglobulin antibody

Beta 2 microglobulin precursor antibody

Beta chain of mhc class 1 proteins antibody

Beta chain of MHC class I molecules antibody

Beta-2-microglobulin form pI 5.3 antibody

CDABP0092 antibody

Hdcma22p antibody

图片

  • Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat spleen tissue lysate<br />Lane 4: Rat liver tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 12 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat spleen tissue lysate
    Lane 4: Rat liver tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 12 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Beta-2 Microglobulin KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa<br /><br />Exposure time: 62 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Beta-2 Microglobulin KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

    Exposure time: 62 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/500 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: U937 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 2 minutes;<br /><br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody (ET1608-45) at 1/500 dilution.

    Lane 1: Raji cell lysate
    Lane 2: U937 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa

    Exposure time: 2 minutes;

    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Beta-2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Beta-2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Beta-2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1608-45" style="font-weight: bold;text-decoration: underline;">ET1608-45</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Beta-2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

同靶点&同通路的产品

metafield image

Beta-2 Microglobulin Rabbit Polyclonal Antibody

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

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