c-Fos Recombinant Antibody [PSH05-77] - Rat IgG1 (Chimeric)
Catalog# HA601373
c-Fos Recombinant Antibody [PSH05-77] - Rat IgG1 (Chimeric)
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IHC-Fr
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IF-Tissue
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Mouse
-
Human
概述
产品名称
c-Fos Recombinant Antibody [PSH05-77] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within human Protein c-Fos aa 1-380.
产品特异性
This antibody detects endogenous levels of total c-Fos protein. The antibody does not cross-react with other Fos proteins.
种属反应性
Mouse, Human
验证应用
IHC-Fr, IF-Tissue
偶联
unconjugated
克隆号
PSH05-77
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IF-Tissue
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1:500
靶点
功能
Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.
背景文献
1. Matsuoka K et al. Metabolic rewiring controlled by c-Fos governs cartilage integrity in osteoarthritis. Ann Rheum Dis. 2023 Sep
2. Osada N et al. c-FOS is an integral component of the IKZF1 transactivator complex and mediates lenalidomide resistance in multiple myeloma. Clin Transl Med. 2023 Aug
亚细胞定位
Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.
别名
Activator protein 1 antibody
AP 1 antibody
C FOS antibody
Cellular oncogene c fos antibody
Cellular oncogene fos antibody
FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
FBJ murine osteosarcoma viral oncogene homolog antibody
FBJ murine osteosarcoma viral v fos oncogene homolog antibody
FBJ Osteosarcoma Virus antibody
FOS antibody
展开图片
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Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-c-Fos antibody (HA601373) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601373, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling c-Fos with Rat anti-c-Fos antibody (HA601373) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601373, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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