iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
Catalog# HA1133
iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
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IF-Cell
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IF-Tissue
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FC
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Rat
概述
产品名称
iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody
抗体类型
Goat Polyclonal Antibody
免疫原
Rat IgG(H+L).
种属反应性
Rat
验证应用
IF-Cell, IF-Tissue, FC
偶联
iFluor™ 488
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Cell
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1:500
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IF-Tissue
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1:500
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FC
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1:500
靶点
功能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.
背景文献
暂无
图片
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Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NCAM1 with Rat anti-NCAM1 antibody at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NCAM1, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"