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GFAP Rabbit Polyclonal Antibody

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Catalog# R1308-9

GFAP Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GFAP Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within N-terminal human GFAP.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P, FC

分子量

Predicted band size: 50 kDa

阳性对照

Human brain tissue lysates, mouse brain tissue lysates, A172, human brain tissue, rat hippocampus tissue.

偶联

unconjugated

RRID

AB_3073239

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

发表文章中的应用

WB See 1 publications below

发表文章中的种属

Mouse See 1 publications below

靶点

功能

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system.

背景文献

1. "A new splice variant of glial fibrillary acidic protein GFAPepsilon, interacts with the presenilin proteins." Nielsen A.L., Holm I.E., Johansen M., Bonven B., Jorgensen P., Jorgensen A.L. J. Biol. Chem. 277:29983-29991(2002)

2. "Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease." Brenner M., Johnson A.B., Boespflug-Tanguy O., Rodriguez D., Goldman J.E., Messing A. Nat. Genet. 27:117-120(2001)

序列相似性

Belongs to the intermediate filament family.

组织特异性

Expressed in cells lacking fibronectin.

翻译后修饰

Phosphorylated by PKN1.

亚细胞定位

Cytoplasm, Intermediate filament.

UNIPROT #

SwissProt: P14136 Human

别名

wu:fb34h11 antibody

ALXDRD antibody

cb345 antibody

etID36982.3 antibody

FLJ42474 antibody

FLJ45472 antibody

GFAP antibody

GFAP_HUMAN antibody

gfapl antibody

Glial fibrillary acidic protein antibody

展开

图片

  • Western blot analysis on human brain tissue lysates using anti-GFAP rabbit polyclonal antibodies.

    Western blot analysis on human brain tissue lysates using anti-GFAP rabbit polyclonal antibodies.

  • Western blot analysis of GFAP on mouse brain tissue lysates with Rabbit anti-GFAP antibody (<a href="/products/R1308-9" style="font-weight: bold;text-decoration: underline;">R1308-9</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1308-9" style="font-weight: bold;text-decoration: underline;">R1308-9</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GFAP on mouse brain tissue lysates with Rabbit anti-GFAP antibody (R1308-9) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescent staining of A172 cells using anti- GFAP rabbit polyclonal antibody.

    Immunofluorescent staining of A172 cells using anti- GFAP rabbit polyclonal antibody.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- GFAP rabbit polyclonal antibody.

    Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- GFAP rabbit polyclonal antibody.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (<a href="/products/R1308-9" style="font-weight: bold;text-decoration: underline;">R1308-9</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/R1308-9" style="font-weight: bold;text-decoration: underline;">R1308-9</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (R1308-9) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

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