HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
Catalog# NBI02H
HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
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WB
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IP
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Mouse
概述
产品名称
HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
抗体类型
Alpaca Monoclonal Antibody
免疫原
Mouse IgG
种属反应性
Mouse
验证应用
WB, IP
偶联
HRP
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at –20 ℃, protect from light.
存储缓冲液
PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol.
亚型
IgG
纯化方式
Recombinant Expression and Affinity purified,Dissociation constant KD of 0.1nM.
应用稀释度
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WB
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1:1,000-1:10,000
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IP
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sample should be completely reduced/denatured before loaded onto a western blot.
靶点
功能
Anti-mouse IgG Nano-secondary antibody for IP (HRP) is based on Monovalent, recombinant single domain antibodies to mouse IgG coupled to HRP, and the Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protien was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protien. Using Anti-mouse IgG for IP Nano-secondary antibody (HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.
背景文献
暂无
图片
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Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation.
Lane1/2: Immunoprecipitating IgG in recombinant protein with flag on N-terminal
Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with flag on N-terminal -
PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA601172 IP in HeLa cell lysate
Lane 3: Mouse IgG instead of HA601172 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds; ECL: K1801 -
PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate (input)
Lane 2: HA601172 IP in NIH/3T3 cell lysate
Lane 3: Mouse IgG instead of HA601172 in NIH/3T3 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"